Henderson Masthead
Downloadable Resources

Automated Contour-based Tracking for In Vitro Environments (ACTIVE)


ACTIVE (Automated Contour-based Tracking for In Vitro Environments) was developed for automated nuclear cell tracking and was created using MATLAB version 2011a.

A detailed explanation of ACTIVE and representative results generated by ACTIVE are available in the original research article: Baker RM, Brasch ME, Manning ML, Henderson JH. Automated, contour-based tracking and analysis of cell behaviour over long time scales in environments of varying complexity and cell density. Journal of The Royal Society Interface. 2014;11(97). doi: 10.1098/rsif.2014.0386.

Conditions and terms of use:
  • Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.
  • End users are asked to cite the following works when giving appropriate credit under the terms of the Creative Commons License (whenever results or derivatives that are based on the software are presented or published):
    • Citation to ACTIVE:
      • Baker RM, Brasch ME, Manning ML, Henderson JH. Automated, contour-based tracking and analysis of cell behaviour over long time scales in environments of varying complexity and cell density. Journal of The Royal Society Interface. 2014;11(97). doi: 10.1098/rsif.2014.0386.
    • Citations to work foundational to ACTIVE:
      • Idema T. A new way of tracking motion, shape, and divisions. European Biophysics Journal. 2013;42(8):647654
      • Crocker JC, Grier DG. Methods of digital video microscopy for colloidal studies. Journal of Colloid and Interface Science. 1996;179(1):298-310.
      • Gao Y, Kilfoil ML. Accurate detection and complete tracking of large populations of features in three dimensions. Optics Express. 2009;17(6):4685-704.
  • Please note that these citations are included in the ACTIVE download in standardized formats (.ris and .bib).
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Development of ACTIVE was supported by the National Science Foundation under an IGERT Fellowship (DGE-1068780) to MEB and Forrest Smith and under an award (CMMI-1334611) to JHH and MLM, and by DARPA under a Young Faculty Award (D12AP00271) to JHH.
Last Updated: 7/21/2014